Establishment of an in vitro method for micropropagation of ironwort, (Sideritis raeseri Boiss. & Heldr.)

Ironwort / Mountain Tea (Sideritis raeseri Boiss & Heldr.) is an endangered (EN) plant species in Albania. This study aimed to develop a rapid clonal propagation protocol using vitro methodologies. The ironwort seeds were pre-treated with three concentrations of GA3 (250, 500, and 1000 mg l-1). During the inoculation stage, two types culture media, Murashige Skoog (MS) Woody Plant Medium (WPM), tested, effects both concentration media used evaluated. For subculture cytokinins (6-benzylaminopurine BAP, kinetin, zeatin) at four (0.5; 1.0; 1.5; 2.0 l-1), compared for RGR index, while rooting different auxins (1-naphthaleneacetic acid NAA indole-3-butyric IBA) l-1) tested. 500 l-1 MS medium resulted as more effective. highest value index during stage was obtained nutrient supplemented BAP 1.5 l-1. rhizogenesis response, IBA effective roots length number. Based on these results, methodologies can be promising tool mass production this possible applications enhancing valuable nutraceuticals.